Study of Oxidative Stress Markers in Experimental Exposure to Isocyanate

Authors

  • Ovidiu Persecă
  • Remus Orăsan
  • Alina Elena PÂrvu
  • Remus Moldovan

Keywords:

isocyanates, asthma, oxidative stress, trolox

Abstract

Aim. Toluene diisocyanate (TDI) is a frequent cause of occupational asthma. The aim of this study was to evaluate the systemic oxidative stress in experimental exposure to TDI.

Methods. An experimental model of mice exposure to TDI was used for 10 days. The study groups (n=12) were: a negative control group with skin sensitization and intranasal challenge, performed with saline; a group with sensitization and challenge with the solvent; a group with sensitization and challenge with TDI; a group with TDI and trolox (20 mg/kg /day ip). On the 11 th day, blood was collected by cardiac puncture, and oxidative stress was assessed by malondialdehyde (MDA), carbonylated proteins (CP), total thiols (TSH) and serum glutathione (GSH).

Results. TDI increased MDA, PC and TSH and reduced GSH. The solvent did not influence any parameter. Trolox reduced PC and increased GSH.

Conclusions. Experimental exposure of mice to TDI caused systemic oxidative stress and Trolox lowered the systemic oxidative stress.

Author Biographies

Ovidiu Persecă, Centrul Regional de Sănătate Publică, Cluj-Napoca

Sănătate Ocupaţională

Remus Orăsan, ”Iuliu Haţieganu” University of Medicine and Pharmacy, Cluj-Napoca

Disciplina de Fiziologie

Alina Elena PÂrvu, ”Iuliu Haţieganu” University of Medicine and Pharmacy, Cluj-Napoca

Disciplina de Fiziopatologie

Remus Moldovan, ”Iuliu Haţieganu” University of Medicine and Pharmacy, Cluj-Napoca

Disciplina de Fiziologie

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Published

2014-02-15

How to Cite

1.
Persecă O, Orăsan R, PÂrvu AE, Moldovan R. Study of Oxidative Stress Markers in Experimental Exposure to Isocyanate. Med Pharm Rep [Internet]. 2014 Feb. 15 [cited 2025 Oct. 6];85(3):358-62. Available from: https://medpharmareports.com/index.php/mpr/article/view/174

Issue

Vol. 85 No. 3 (2012)

Section

Original Research

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