Study of Oxidative Stress Markers in Experimental Exposure to Isocyanate
Abstract
Aim. Toluene diisocyanate (TDI) is a frequent cause of occupational asthma. The aim of this study was to evaluate the systemic oxidative stress in experimental exposure to TDI.
Methods. An experimental model of mice exposure to TDI was used for 10 days. The study groups (n=12) were: a negative control group with skin sensitization and intranasal challenge, performed with saline; a group with sensitization and challenge with the solvent; a group with sensitization and challenge with TDI; a group with TDI and trolox (20 mg/kg /day ip). On the 11 th day, blood was collected by cardiac puncture, and oxidative stress was assessed by malondialdehyde (MDA), carbonylated proteins (CP), total thiols (TSH) and serum glutathione (GSH).
Results. TDI increased MDA, PC and TSH and reduced GSH. The solvent did not influence any parameter. Trolox reduced PC and increased GSH.
Conclusions. Experimental exposure of mice to TDI caused systemic oxidative stress and Trolox lowered the systemic oxidative stress.