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Copyright
© Clujul Medical, 2019
Affiliations
Mihai Porojan
UMF Cluj
Cornelia Bala
UMF Cluj
Roxana Ilies
UMF Cluj
Andreea Catana
UMF Cluj
Radu A. Popp
UMF Cluj
Dan L. Dumitrascu
UMF Cluj
How to Cite
Combined glutathione S transferase M1/T1 null genotypes is associated with type 2 Diabetes mellitus
Abstract
Background
Due to new genetic insights, a considerably large number of genes and polymorphic gene variants are screened and linked with the complex pathogenesis of type 2 diabetes (DM).
Our study aimed to investigate the association between the two isoforms of the glutathione S-transferase genes (Glutathione S transferase isoemzyme type M1- GSTM1 and Glutathione S transferase isoemzyme type T1-GSTT1) and the prelevance of DM in the Northern Romanian population.
Methods
We conducted a cross-sectional, randomized, case-control study evaluating the frequency of GSTM1 and GSTT1 null alleles in patients diagnosed with DM. A total of 106 patients diagnosed with DM and 124 healthy controls were included in the study. GSTM1 and GSTT1 null alleles genotyping was carried out using Multiplex PCR amplification of relevant gene fragments, followed by gel electrophoresis analysis of the resulting amplicons.
Results
Molecular analysis did not reveal an increased frequency of the null GSTM1, and GSTT1 alleles (mutant genotypes) respectively in the DM group compared to controls (p=0.171, OR=1.444 CI=0.852-2.447; p=0.647, OR=0.854, CI=0.436-1.673). Nevertheless, the combined GSTM1/GSTT1 null genotypes were statistically significantly higher in DM patients compared to control subjects (p=0.0021, OR=0.313, CI=0.149-0.655)
Conclusions
The main finding of our study is that the combined, double GSTM1/GSTT1 null genotypes are to be considered among the polymorphic genetic risk factors for type 2 DM.