ASSESSMENT OF ISOFLAVONE AGLYCONES VARIABILITY IN SOY FOOD SUPPLEMENTS USING A VALIDATED HPLC-UV METHOD

Authors

DOI:

https://doi.org/10.15386/cjmed-468

Keywords:

food supplement, genistein, HPLC, isoflavones, standardization

Abstract

Background and aims. Soy supplements are often recommended in the management of menopause symptoms. The declared content of soy supplements is commonly expressed as total isoflavones per dosage form. Given that soy isoflavones have different estrogenic potencies, pharmacokinetics and metabolism, the aim of this study was to evaluate the total isoflavone content and the aglycone profile of seven soy supplements and one soy seed extract. Label accuracy was assessed, in relation to the precise content and the recommended posology for estimating whether the optimal dose is achieved for alleviating menopause symptoms.

Materials and Methods. A high performance liquid chromatography method was developed for evaluating the aglycone content (genistein, daidzein, glycitein). After extraction and acidic hydrolysis, the aglycones were separated on a C18 column, using 0.1% acetic acid and acetonitrile as mobile phases. The flow rate was 1.5mL min-1 and the UV detector wavelength was set at 260nm. A linear relationship was found in the range 5-80µg mL-1. The method was validated using the accuracy profile methodology.

Results and Discussions. The total isoflavone content ranged from 6.07 to 41.68mg dosage form-1. Various aglycone profiles were obtained for each supplement which can result in a different estrogenic activity, bioavailability and finally, in a different efficiency in alleviating menopause symptoms. In most clinical trials where soy isoflavones were evaluated, little attention was paid to determining the exact aglycone profile of the employed soy extracts.

Conclusions. As clinical outcomes continue to be controversial, this study highlights the need of standardization in genistein, rather than total isoflavones and labeling accuracy for soy supplements.

Author Biographies

Alina Uifălean, Faculty of Pharmacy, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj Napoca

Department of Pharmaceutical Analysis

Anca Farcaş, Faculty of Pharmacy, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj Napoca

Department of Mathematics and Computer Science

Maria Ilieş, Faculty of Pharmacy, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj Napoca

Department of Pharmaceutical Analysis

Simona Codruţa Hegheş, Faculty of Pharmacy, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj Napoca

Department of Pharmaceutical Analysis

Corina Ionescu, Faculty of Pharmacy, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj Napoca

Department of Pharmaceutical Biochemistry and Clinical Laboratory

Cristina Adela Iuga, Faculty of Pharmacy, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj Napoca

Department of Pharmaceutical Analysis

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Published

2015-06-19

How to Cite

1.
Uifălean A, Farcaş A, Ilieş M, Hegheş SC, Ionescu C, Iuga CA. ASSESSMENT OF ISOFLAVONE AGLYCONES VARIABILITY IN SOY FOOD SUPPLEMENTS USING A VALIDATED HPLC-UV METHOD. Med Pharm Rep [Internet]. 2015 Jun. 19 [cited 2025 Oct. 5];88(3):373-80. Available from: https://medpharmareports.com/index.php/mpr/article/view/468

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Section

Original Research