Micro-CT and histological analysis of Ti6Al7Nb custom made implants with hydroxyapatite and SiO2-TiO2 coatings in a rabbit model

Authors

  • Gabriel Armencea
  • Cristian Berce
  • Horatiu Rotaru
  • Simion Bran
  • Dan Leordean
  • Camelia Coada
  • Milica Todea
  • Camelia Augusta Jula
  • Dan Gheban
  • Grigore Baciut
  • Mihaela Baciut
  • Radu Septimiu Campian

DOI:

https://doi.org/10.15386/cjmed-479

Keywords:

micro-CT, Ti6Al7Nb, SLM, osseointegration, implant coating, custom made implant

Abstract

Background and aim. Bone defect reconstruction in the maxillofacial area comes as a necessity after traumatic, oncological or congenital pathology. Custom made implant manufacturing, such as selective laser melting (SLM), is very helpful when bone reconstruction is needed. In the present study we assessed the osseointegration of custom made implants made of Ti6Al7Nb with two different coatings: SiO2-TiO2 and hydroxyapatite, by comparing the bone mineral density (BMD) measured on micro-CT and the histological mineralized bone surrounding the implants.

Methods. Custom made – cylindrical type – implants were produced by selective laser melting, coated with SiO2-TiO2 and hydroxyapatite and implanted in the rabbit femur. The animals (divided into 3 groups) were sacrificed at 1, 3 and 6 months and the implants were removed together with the surrounding bone. Bone mineral density and histological examination of the bone-implant surface was performed for each group.

Results. BMD and histological examination of the samples determined the quantity of mineralized bone at the implant site, showing a good percentage of mineralized bone for the coated implants at 1, 3 and 6 months,  The measurements for the implants without coating showed a significant lower quantity of mineralized bone at 3 months compared with the implants with coating, and a good quantity of mineralized bone at 6 months, showing a process of demineralization followed by remineralization in the last month. The measurements of BMD showed similar results with the histological examination.

Conclusions. The use of micro-CT and the measurement of BMD are a reliable, minimally invasive and a quick method of osseointegration assessment.

Author Biographies

Gabriel Armencea, Department of Oral and Maxillofacial Surgery, UMF “Iuliu-Hatieganu”, Cluj-Napoca

Department of Oral and Maxillofacial Surgery

Cristian Berce, Laboratory Animal Facility, UMF “Iuliu-Hatieganu”, Cluj-Napoca

Laboratory Animal Facility

Horatiu Rotaru, Department of Oral and Maxillofacial Surgery, UMF “Iuliu-Hatieganu”, Cluj-Napoca

Department of Oral and Maxillofacial Surgery

Simion Bran, Department of Oral and Maxillofacial Surgery, UMF “Iuliu-Hatieganu”, Cluj-Napoca

Department of Oral and Maxillofacial Surgery

Dan Leordean, Department of Manufacturing Engineering, Technical University, Cluj-Napoca

  1. Department of Manufacturing Engineering

Camelia Coada, Faculty of Medicine, University of Medicine and Pharmacy “Iuliu-Hatieganu”, Cluj-Napoca

Faculty of Medicine

Milica Todea, Faculty of Physics & Institute of Interdisciplinary Research in Bio-Nano-Sciences, Babes Bolyai University

Institute of Interdisciplinary Research in
Bio-Nano-Sciences

Camelia Augusta Jula, Student, Faculty of Dental Medicine, UMF “Iuliu Hatieganu”, Cluj-Napoca

Faculty of Dental Medicine

Dan Gheban, Department of Pathology, UMF “Iuliu Hatieganu”, Cluj-Napoca

Department of  Pathology

Grigore Baciut, Department of Oral and Maxillofacial Surgery, UMF “Iuliu-Hatieganu”, Cluj-Napoca

Department of Oral and Maxillofacial Surgery

Mihaela Baciut, Department of Oral and Maxillofacial Surgery, UMF “Iuliu-Hatieganu”, Cluj-Napoca

Department of Oral and Maxillofacial Surgery

Radu Septimiu Campian, Department of Oral Rehabilitation, Oral Health and Management of Dental Office, UMF “Iuliu-Hatieganu”, Cluj-Napoca

Department of Oral Rehabilitation, Oral Health and Management of Dental Office

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Published

2015-06-19

How to Cite

1.
Armencea G, Berce C, Rotaru H, Bran S, Leordean D, Coada C, Todea M, Jula CA, Gheban D, Baciut G, Baciut M, Campian RS. Micro-CT and histological analysis of Ti6Al7Nb custom made implants with hydroxyapatite and SiO2-TiO2 coatings in a rabbit model. Med Pharm Rep [Internet]. 2015 Jun. 19 [cited 2025 Oct. 5];88(3):408-14. Available from: https://medpharmareports.com/index.php/mpr/article/view/479

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Section

Original Research